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A tropomyosin-2 mutation suppresses a troponin I myopathy in Drosophila

Naimi, B., Harrison, A., Cummins, M. and Nongthomba, U. (2001) A tropomyosin-2 mutation suppresses a troponin I myopathy in Drosophila. Molecular Biology of the Cell, 12 (5). pp. 1529-1539. ISSN 1059-1524

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A suppressor mutation, D53, of the held-up2 allele of the Drosophila melanogaster Troponin I (wupA) gene is described. D53, a missense mutation, S185F, of the tropomyosin-2, Tm2, gene fully suppresses all the phenotypic effects of held-up2, including the destructive hypercontraction of the indirect flight muscles (IFMs), a lack of jumping, the progressive myopathy of the walking muscles, and reductions in larval crawling and feeding behavior. The suppressor restores normal function of the IFMs, but flight ability decreases with age and correlates with an unusual, progressive structural collapse of the myofibrillar lattice starting at the center. The S185F substitution in Tm2 is close to a troponin T binding site on tropomyosin. Models to explain suppression by D53, derived from current knowledge of the vertebrate troponin-tropomyosin complex structure and functions, are discussed. The effects of S185F are compared with those of two mutations in residues 175 and 180 of human -tropomyosin 1 which cause familial hypertrophic cardiomyopathy (HCM).

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 26 Mar 2009 10:32
Last Modified: 26 Mar 2009 10:32
Published Version: http://www.molbiolcell.org/cgi/content/abstract/12...
Status: Published
Publisher: American Society for Cell Biology
URI: http://eprints.whiterose.ac.uk/id/eprint/7035

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