White Rose University Consortium logo
University of Leeds logo University of Sheffield logo York University logo

Trypanosoma brucei ARF1 Plays a Central Role in Endocytosis and Golgi-Lysosome Trafficking

Price, H. P., Stark, M. and Smith, D. F. (2007) Trypanosoma brucei ARF1 Plays a Central Role in Endocytosis and Golgi-Lysosome Trafficking. Molecular Biology of the Cell, 18 (3). pp. 864-873. ISSN 1059-1524

Full text not available from this repository.

Abstract

The ADP ribosylation factor (Arf)1 orthologue in the divergent eukaryote Trypanosoma brucei (Tb) shares characteristics with both Arf1 and Arf6 and has a vital role in intracellular protein trafficking. TbARF1 is Golgi localized in trypanosomes but associates with the plasma membrane when expressed in human cells. Depletion of TbARF1 by RNA interference causes a major decrease in endocytosis, which correlates with Rab5 dissociation from early endosomes. Although the Golgi remains intact, parasites display enlarged flagellar pockets and intracellular flagella. An increase in active GTP-bound TbARF1 in bloodstream parasites is rapidly lethal, correlating with a defect in Golgi-to-lysosome transport. We conclude that the essential Golgi-localizing T. brucei ARF1 has a primary role in the maintenance of both post-Golgi transport and endocytosis and that it is significantly divergent from other characterized ARFs.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 16 Mar 2009 16:53
Last Modified: 16 Mar 2009 16:53
Published Version: http://dx.doi.org/10.1091/mbc.E06-08-0736
Status: Published
Publisher: American Society for Cell Biology
Identification Number: 10.1091/mbc.E06-08-0736
URI: http://eprints.whiterose.ac.uk/id/eprint/7032

Actions (repository staff only: login required)