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Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism

Benini, S., Rypniewski, W.R., Wilson, K.S., Mangani, S. and Ciurli, S. (2004) Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism. Journal of the American Chemical Society, 126 (12). pp. 3714-3715. ISSN 0002-7863

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Abstract

The structure of the complex of urease, a Ni-containing metalloenzyme, with boric acid was determined at 2.10 A resolution. The complex shows the unprecedented binding mode of the competitive inhibitor to the dinuclear metal center, with the B(OH)3 molecule bridging the Ni ions and leaving in place the bridging hydroxide. Boric acid can be considered a substrate analogue of urea, and the structure supports the proposal that the Ni-bridging hydroxide acts as the nucleophile in the enzymatic process of urea hydrolysis.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 03 Apr 2009 11:13
Last Modified: 03 Apr 2009 11:13
Published Version: http://dx.doi.org/10.1021/ja049618p
Status: Published
Publisher: ACS American Chemical Society
Identification Number: 10.1021/ja049618p
URI: http://eprints.whiterose.ac.uk/id/eprint/6886

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