Smalley, M.J., Signoret, N., Robertson, D., Tilley, A., Hann, A., Ewan, K., Ding, Y., Paterson, H. and Dale, T.C. (2005) Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta. Journal of Cell Science, 118 (22). pp. 5279-5289. ISSN 0021-9533
Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.
|Institution:||The University of York|
|Academic Units:||The University of York > Hull York Medical School (York)|
|Depositing User:||York RAE Import|
|Date Deposited:||24 Apr 2009 11:07|
|Last Modified:||24 Apr 2009 11:07|
|Publisher:||Company of Biologist Ltd|