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Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta

Smalley, M.J., Signoret, N., Robertson, D., Tilley, A., Hann, A., Ewan, K., Ding, Y., Paterson, H. and Dale, T.C. (2005) Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta. Journal of Cell Science, 118 (22). pp. 5279-5289. ISSN 0021-9533

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Abstract

Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.

Item Type: Article
Academic Units: The University of York > Hull York Medical School (York)
Depositing User: York RAE Import
Date Deposited: 24 Apr 2009 11:07
Last Modified: 24 Apr 2009 11:07
Published Version: http://dx.doi.org/10.1242/jcs.02647
Status: Published
Publisher: Company of Biologist Ltd
Identification Number: 10.1242/jcs.02647
URI: http://eprints.whiterose.ac.uk/id/eprint/6532

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