Smalley, M.J., Signoret, N., Robertson, D., Tilley, A., Hann, A., Ewan, K., Ding, Y., Paterson, H. and Dale, T.C. (2005) Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta. Journal of Cell Science, 118 (22). pp. 5279-5289. ISSN 0021-9533
Full text not available from this repository.Abstract
Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.
| Item Type: | Article |
|---|---|
| Academic Units: | The University of York > Hull York Medical School (York) |
| Depositing User: | York RAE Import |
| Date Deposited: | 24 Apr 2009 11:07 |
| Last Modified: | 24 Apr 2009 11:07 |
| Published Version: | http://dx.doi.org/10.1242/jcs.02647 |
| Status: | Published |
| Publisher: | Company of Biologist Ltd |
| Identification Number: | 10.1242/jcs.02647 |
| URI: | http://eprints.whiterose.ac.uk/id/eprint/6532 |
Actions (login required)
 |
View Item |
