White Rose University Consortium logo
University of Leeds logo University of Sheffield logo York University logo

The iron-binding properties of aminochelin, the mono(catecholamide) siderophore of Azotobacter vinelandii

Khodr, H.H., Hider, R.C. and Duhme-Klair, A.-K. (2002) The iron-binding properties of aminochelin, the mono(catecholamide) siderophore of Azotobacter vinelandii. Journal of Biological Inorganic Chemistry, 7 (7-8). pp. 891-896. ISSN 0949-8257

Full text not available from this repository.


Azotobacter vinelandii produces siderophores with different metal-binding properties, depending on the concentration of Fe(III) and molybdate in the growth medium. The three protonation constants of the mono(catecholamide) siderophore aminochelin were determined by simultaneous spectrophotometric and potentiometric titrations as log K 1=12.1, log K 2=10.22 and log K 3=7.04. Based on the two catechol protonation constants, log K 1 and log K 3, the overall stability constant of the aminochelin iron 3:1 complex was found to be log ß 3=41.3, resulting in a pFe 3+ value of 17.6 at pH 7.45. In order to further investigate the properties of the siderophore, the solubilization of Fe(III) hydroxide by a 8×10 –4 M solution of aminochelin at pH 7 and 25 °C was followed spectrophotometrically in the absence and in the presence of molybdate. It was observed that the addition of molybdate resulted in a significant delay in the solubilization.

Item Type: Article
Keywords: Aminochelin ; Azotobacter vinelandii ; Catecholamide ; Siderophore
Institution: The University of York
Academic Units: The University of York > Chemistry (York)
Depositing User: York RAE Import
Date Deposited: 29 May 2009 10:31
Last Modified: 29 May 2009 10:31
Published Version: http://dx.doi.org/10.1007/s00775-002-0375-x
Status: Published
Publisher: Springer Science + Business Media
Refereed: Yes
Identification Number: 10.1007/s00775-002-0375-x
URI: http://eprints.whiterose.ac.uk/id/eprint/6525

Actions (repository staff only: login required)