Lebedev, A.A., Krause, M.H., Isidro, A.L., Vagin, A.A., Orlova, E.V., Turner, J., Dodson, E.J., Tavares, P. and Antson, A.A. (2007) Structural framework for DNA translocation via the viral portal protein. EMBO Journal, 26 (7). pp. 1984-1994. ISSN 0261-4189Full text not available from this repository.
Tailed bacteriophages and herpesviruses load their capsids with DNA through a tunnel formed by the portal protein assembly. Here we describe the X-ray structure of the bacteriophage SPP1 portal protein in its isolated 13-subunit form and the pseudoatomic structure of a 12-subunit assembly. The first defines the DNA-interacting segments (tunnel loops) that pack tightly against each other forming the most constricted part of the tunnel; the second shows that the functional dodecameric state must induce variability in the loop positions. Structural observations together with geometrical constraints dictate that in the portal–DNA complex, the loops form an undulating belt that fits and tightly embraces the helical DNA, suggesting that DNA translocation is accompanied by a 'mexican wave' of positional and conformational changes propagating sequentially along this belt.
|Institution:||The University of York|
|Academic Units:||The University of York > Chemistry (York)|
|Depositing User:||York RAE Import|
|Date Deposited:||11 Jun 2009 13:25|
|Last Modified:||11 Jun 2009 13:25|
|Publisher:||Nature Publishing Group|