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Bacterial DNA segregation dynamics mediated by the polymerizing protein ParF

Barillà, D., Rosenberg, M.F., Nobbmann, U. and Hayes, F. (2005) Bacterial DNA segregation dynamics mediated by the polymerizing protein ParF. EMBO Journal, 24 (7). pp. 1453-1464. ISSN 0261-4189

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Abstract

Prokaryotic DNA segregation most commonly involves members of the Walker-type ParA superfamily. Here we show that the ParF partition protein specified by the TP228 plasmid is a ParA ATPase that assembles into extensive filaments in vitro. Polymerization is potentiated by ATP binding and does not require nucleotide hydrolysis. Analysis of mutations in conserved residues of the Walker A motif established a functional coupling between filament dynamics and DNA partitioning. The partner partition protein ParG plays two separable roles in the ParF polymerization process. ParF is unrelated to prokaryotic polymerizing proteins of the actin or tubulin families, but is a homologue of the MinD cell division protein, which also assembles into filaments. The ultrastructures of the ParF and MinD polymers are remarkably similar. This points to an evolutionary parallel between DNA segregation and cytokinesis in prokaryotic cells, and reveals a potential molecular mechanism for plasmid and chromosome segregation mediated by the ubiquitous ParA-type proteins.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 29 May 2009 15:21
Last Modified: 29 May 2009 15:21
Published Version: http://dx.doi.org/10.1038/sj.emboj.7600619
Status: Published
Publisher: Nature Publishing Group
Identification Number: 10.1038/sj.emboj.7600619
URI: http://eprints.whiterose.ac.uk/id/eprint/6046

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