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Real-time image acquisition for absorbance detection and quantification in thin-layer chromatography

Lancaster, M., Goodall, D.M., Bergström, E.T., McCrossen, S. and Myers, P. (2006) Real-time image acquisition for absorbance detection and quantification in thin-layer chromatography. Analytical Chemistry, 78 (3). pp. 905-911. ISSN 0003-2700

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This paper presents the first quantitative study of real-time acquisition of images of spots on thin-layer chromatographic plates during development. Procedures are described for imaging using a CCD camera and for image processing, incorporating corrections for fixed pattern effects and compensation for the moving solvent front, to measure the absorbance of the analyte. Imaging of Sudan II was carried out in transmission mode, and peak areas were found to be time-independent. Quantification of the relationship between peak area and sample loading was established over the range 1−50 ng. After averaging 55 images obtained during a single chromatographic run, which attenuates noise contributions from local nonuniformities in the sorbent layer, precision and detection limits were found to be comparable with values obtained in previous work using offline measurements.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Chemistry (York)
Depositing User: York RAE Import
Date Deposited: 21 Aug 2009 11:06
Last Modified: 21 Aug 2009 11:06
Published Version: http://dx.doi.org/10.1021/ac051390g
Status: Published
Publisher: American Chemical Society
Identification Number: 10.1021/ac051390g
URI: http://eprints.whiterose.ac.uk/id/eprint/5533

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