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Delineation of a unique protein-protein interaction site on the surface of the estrogen receptor

Kong, E H, Heldring, N, Gustafsson, J A, Treuter, E, Hubbard, R E and Pike, A C W (2005) Delineation of a unique protein-protein interaction site on the surface of the estrogen receptor. Proceedings of the National Academy of Sciences, USA. pp. 3593-3598. ISSN 0027-8424

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Abstract

Recent studies have identified a series of estrogen receptor (ER)interacting peptides that recognize sites that are distinct from the classic coregulator recruitment (AF2) region. Here, we report the structural and functional characterization of an ER alpha-specific peptide that binds to the liganded receptor in an AF2-independent manner. The 2-angstrom crystal structure of the ER/peptide complex reveals a binding site that is centered on a shallow depression on the beta-hairpin face of the ligand-binding domain. The peptide binds in an unusual extended conformation and makes multiple contacts with the ligand-binding domain. The location and architecture of the binding site provides an insight into the peptide's ER subtype specificity and ligand interaction preferences. In vivo, an engineered coactivator containing the peptide motif is able to strongly enhance the transcriptional activity of liganded ER alpha, particularly in the presence of 4-hydroxytamoxifen. Furthermore, disruption of this binding surface alters ER's response to the coregulator TIF2. Together, these results indicate that this previously unknown interaction site represents a bona fide control surface involved in regulating receptor activity.

Item Type: Article
Copyright, Publisher and Additional Information: © 2005 by The National Academy of Sciences of the USA
Keywords: coregulator, phage display, structure, LIGAND-BINDING DOMAIN, NUCLEAR RECEPTOR, TRANSCRIPTIONAL ACTIVATION, PEPTIDE ANTAGONISTS, TISSUE-SPECIFICITY, STRUCTURAL BASIS, ER-ALPHA, COACTIVATOR, RECRUITMENT, MOTIF
Academic Units: The University of York > Chemistry (York)
Depositing User: Repository Officer
Date Deposited: 11 Jul 2005
Last Modified: 17 Oct 2013 14:23
Published Version: http://dx.doi.org/10.1073/pnas.0407189102
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/553

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