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Effect of 50 Hz Electromagnetic Fields on the Induction of Heat-Shock Protein Gene Expression in Human Leukocytes

Coulton, L.A., Harris, P.A., Barker, A.T. and Pockley, A.G. (2004) Effect of 50 Hz Electromagnetic Fields on the Induction of Heat-Shock Protein Gene Expression in Human Leukocytes. Radiation Research, 161 (4). pp. 430-434. ISSN 0033-7587

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Abstract

Although evidence is controversial, exposure to environmental power-frequency magnetic fields is of public concern. Cells respond to some abnormal physiological conditions by producing cytoprotective heat-shock (or stress) proteins. In this study, we determined whether exposure to power-frequency magnetic fields in the range 0–100 μT rms either alone or concomitant with mild heating induced heat-shock protein gene expression in human leukocytes, and we compared this response to that induced by heat alone. Samples of human peripheral blood were simultaneously exposed to a range of magnetic-field amplitudes using a regimen that was designed to allow field effects to be distinguished from possible artifacts due to the position of the samples in the exposure system. Power-frequency magnetic-field exposure for 4 h at 37°C had no detectable effect on expression of the genes encoding HSP27, HSP70A or HSP70B, as determined using reverse transcriptase-PCR, whereas 2 h at 42°C elicited 10-, 5- and 12-fold increases, respectively, in the expression of these genes. Gene expression in cells exposed to power-frequency magnetic fields at 40°C was not increased compared to cells incubated at 40°C without field exposure. These findings and the extant literature suggest that power-frequency electromagnetic fields are not a universal stressor, in contrast to physical agents such as heat.

Item Type: Article
Copyright, Publisher and Additional Information: © 2004 by Radiation Research Society.
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine (Sheffield) > Clinical Sciences Division North (Sheffield)
Depositing User: Repository Officer
Date Deposited: 27 Jun 2005
Last Modified: 07 Jun 2014 07:18
Published Version: http://www.bioone.org/pdfserv/i0033-7587-161-04-04...
Status: Published
Refereed: Yes
URI: http://eprints.whiterose.ac.uk/id/eprint/519

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