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Micrografting techniques for testing long-distance signalling in Arabidopsis

Turnbull, C.G.N., Booker, J.P. and Leyser, H.M.O. (2002) Micrografting techniques for testing long-distance signalling in Arabidopsis. The Plant Journal, 32 (2). pp. 255-262. ISSN 0960-7412

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Grafting in species other than Arabidopsis has generated persuasive evidence for long-distance signals involved in many plant processes, including regulation of flowering time and shoot branching. Hitherto, such approaches in Arabidopsis have been hampered by the lack of suitable grafting techniques. Here, a range of micrografting methods for young Arabidopsis seedlings are described. The simplest configuration was a single-hypocotyl graft, constructed with or without a supporting collar, allowing tests of root–shoot communication. More complex two-shoot grafts were also constructed, enabling tests of shoot–shoot communication. Integrity of grafts and absence of adventitious roots on scions were assessed using plants constitutively expressing a GUS gene as one graft partner. Using the max1 (more axillary growth) and max3 increased branching mutants, it was shown that a wild-type (WT) rootstock was able to inhibit rosette branching of mutant shoots. In two-shoot grafts with max1 and WT shoots on a max1 rootstock, the mutant shoot branched profusely, but the WT one did not. In two-shoot grafts with max1 and WT shoots on a WT rootstock, neither shoot exhibited increased branching. The results mirror those previously demonstrated in equivalent grafting experiments with the ramosus mutants in pea, and are consistent with the concept that a branching signal is capable of moving from root to shoot, but not from shoot to shoot. These grafting procedures will be valuable for revealing genes associated with many other long-distance signalling pathways, including flowering, systemic resistance and abiotic stress responses.

Item Type: Article
Copyright, Publisher and Additional Information: Open access copy available from the journal web site.
Keywords: Arabidopsis thaliana, max mutants, grafting, shoot branching, long-distance signal
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: Open Access From Journal
Date Deposited: 22 Dec 2008 19:10
Last Modified: 22 Dec 2008 19:10
Published Version: http://dx.doi.org/10.1046/j.1365-313X.2002.01419.x
Status: Published
Publisher: Wiley-Blackwell
Refereed: Yes
Identification Number: 10.1046/j.1365-313X.2002.01419.x
URI: http://eprints.whiterose.ac.uk/id/eprint/5099

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