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The mitochondrial electron transfer flavoprotein complex is essential for survival of Arabidopsis in extended darkness

Ishizaki, K., Schauer, N., Larson, T.R., Graham, I.A., Fernie, A.R. and Leaver, C.J. (2006) The mitochondrial electron transfer flavoprotein complex is essential for survival of Arabidopsis in extended darkness. The Plant Journal, 47 (5). pp. 751-760. ISSN 0960-7412

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Abstract

In mammals, the electron transfer flavoprotein (ETF) is a heterodimeric protein composed of two subunits, α and β, that is responsible for the oxidation of at least nine mitochondrial matrix flavoprotein dehydrogenases. Electrons accepted by ETF are further transferred to the main respiratory chain via the ETF ubiquinone oxide reductase (ETFQO). Sequence analysis of the unique Arabidopsis homologues of two subunits of ETF revealed their high similarity to both subunits of the mammalian ETF. Yeast two-hybrid experiments showed that the Arabidopsis ETFα and ETFβ can form a heteromeric protein. Isolation and characterization of two independent T-DNA insertional Arabidopsis mutants of the ETFβ gene revealed accelerated senescence and early death compared to wild-type during extended darkness. Furthermore in contrast to wild-type, the etfb mutants demonstrated a significant accumulation of several amino acids, isovaleryl CoA and phytanoyl CoA during dark-induced carbohydrate deprivation. These phenotypic characteristics of etfb mutants are broadly similar to those that we observed previously in Arabidopsis etfqo mutants, suggesting functional association between ETF and ETFQO in Arabidopsis, and confirming the essential roles of the ETF/ETFQO electron transfer complex in the catabolism of leucine and involvement in the chlorophyll degradation pathway activated during dark-induced carbohydrate deprivation.

Item Type: Article
Copyright, Publisher and Additional Information: Open access copy available from the journal web site.
Keywords: Arabidopsis thaliana, electron transfer flavoprotein (ETF), carbohydrate deprivation, mitochondria, isovaleryl CoA, phytanoyl CoA
Academic Units: The University of York > Biology (York)
Depositing User: Open Access From Journal
Date Deposited: 23 Dec 2008 12:15
Last Modified: 23 Dec 2008 12:15
Published Version: http://dx.doi.org/10.1111/j.1365-313X.2006.02826.x
Status: Published
Publisher: Wiley-Blackwell
Refereed: Yes
Identification Number: 10.1111/j.1365-313X.2006.02826.x
URI: http://eprints.whiterose.ac.uk/id/eprint/5061

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