Robinson, S., Warburton, K., Seymour, M., Clench, M. and Thomas-Oates, J. (2007) Localization of water-soluble carbohydrates in wheat stems using imaging matrix-assisted laser desorption ionization mass spectrometry. New Phytologist, 173 (2). pp. 438-444. ISSN 0028-646X
• The pool of endogenous water-soluble oligosaccharides found in the stems of wheat (Triticum aestivum) is being investigated as a potential indicator of grain yield. Techniques such as liquid chromatography with mass spectrometry (LC-MS) can profile these analytes but provide no spatial information regarding their distribution in the wheat stem. The imaging matrix-assisted laser desorption ionization (MALDI) mass spectrometry technique has not been utilized for the analysis of oligosaccharides in plant systems previously.
• Imaging MALDI mass spectrometry was used to analyse cross and longitudinal sections from the stems of Triticum aestivum.
• A range of oligosaccharides up to Hex11 were observed. Water-soluble oligosaccharides were ionized as potassiated molecules, and found to be located in the stem pith that is retained predominantly around the inner stem wall.
• Imaging MALDI analyses provided spatial information on endogenous oligosaccharides present in wheat stems. The technique was found to offer comparable sensitivities for oligosaccharide detection to those of our established LC-MS method, and has potential for broad application in studying the in situ localization of other compound types in plant material.
|Copyright, Publisher and Additional Information:||Open access copy available from the journal web site.|
|Keywords:||carbohydrates, imaging, mass spectrometry, matrix-assisted laser desorption ionization (MALDI), oligosaccharide, stem, wheat (Triticum aestivum)|
|Institution:||The University of York|
|Academic Units:||The University of York > Chemistry (York)|
|Depositing User:||Open Access From Journal|
|Date Deposited:||23 Dec 2008 12:28|
|Last Modified:||23 Dec 2008 12:28|