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Localization of water-soluble carbohydrates in wheat stems using imaging matrix-assisted laser desorption ionization mass spectrometry

Robinson, S., Warburton, K., Seymour, M., Clench, M. and Thomas-Oates, J. (2007) Localization of water-soluble carbohydrates in wheat stems using imaging matrix-assisted laser desorption ionization mass spectrometry. New Phytologist, 173 (2). pp. 438-444. ISSN 0028-646X

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Abstract

• The pool of endogenous water-soluble oligosaccharides found in the stems of wheat (Triticum aestivum) is being investigated as a potential indicator of grain yield. Techniques such as liquid chromatography with mass spectrometry (LC-MS) can profile these analytes but provide no spatial information regarding their distribution in the wheat stem. The imaging matrix-assisted laser desorption ionization (MALDI) mass spectrometry technique has not been utilized for the analysis of oligosaccharides in plant systems previously.

• Imaging MALDI mass spectrometry was used to analyse cross and longitudinal sections from the stems of Triticum aestivum.

• A range of oligosaccharides up to Hex11 were observed. Water-soluble oligosaccharides were ionized as potassiated molecules, and found to be located in the stem pith that is retained predominantly around the inner stem wall.

• Imaging MALDI analyses provided spatial information on endogenous oligosaccharides present in wheat stems. The technique was found to offer comparable sensitivities for oligosaccharide detection to those of our established LC-MS method, and has potential for broad application in studying the in situ localization of other compound types in plant material.

Item Type: Article
Copyright, Publisher and Additional Information: Open access copy available from the journal web site.
Keywords: carbohydrates, imaging, mass spectrometry, matrix-assisted laser desorption ionization (MALDI), oligosaccharide, stem, wheat (Triticum aestivum)
Academic Units: The University of York > Chemistry (York)
Depositing User: Open Access From Journal
Date Deposited: 23 Dec 2008 12:28
Last Modified: 23 Dec 2008 12:28
Published Version: http://dx.doi.org/10.1111/j.1469-8137.2006.01934.x
Status: Published
Publisher: Wiley-Blackwell
Refereed: Yes
Identification Number: 10.1111/j.1469-8137.2006.01934.x
URI: http://eprints.whiterose.ac.uk/id/eprint/5058

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