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The Bacillus cereus GerN and GerT protein homologs have distinct roles in spore germination and outgrowth, respectively

Senior, A. and Moir, A. (2008) The Bacillus cereus GerN and GerT protein homologs have distinct roles in spore germination and outgrowth, respectively. Journal of Bacteriology, 190 (18). pp. 6148-6152. ISSN 0021-9193

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Abstract

The GerT protein of Bacillus cereus shares 74% amino acid identity with its homolog GerN. The latter is a Na+/H+-K+ 19 antiporter that is required for normal spore germination in inosine. The germination properties of single and double mutants of B. cereus ATCC10876 reveal that unlike GerN, which is required for all germination responses that involve the GerI germinant receptor, the GerT protein does not have a significant role in germination, although it is required for the residual GerI-mediated inosine germination response of a gerN mutant. In contrast, GerT has a significant role in outgrowth; gerT mutant spores do not outgrow efficiently under alkaline conditions, and outgrow more slowly than wild type in the presence of high NaCl concentrations. The GerT protein in B. cereus therefore contributes to the success of spore outgrowth from the germinated state during alkaline or Na+ stress.

Item Type: Article
Copyright, Publisher and Additional Information: © 2008 American Society for Microbiology. This is an author produced version of a paper subsequently published in Journal of Bacteriology. Uploaded in accordance with the publisher's self-archiving policy.
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield)
Depositing User: Repository Officer
Date Deposited: 07 Oct 2008 09:59
Last Modified: 08 Feb 2013 16:56
Published Version: http://dx.doi.org/10.1128/JB.00789-08
Status: Published
Publisher: American Society for Microbiology
Refereed: Yes
Identification Number: 10.1128/JB.00789-08
URI: http://eprints.whiterose.ac.uk/id/eprint/4718

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