Seavers, P R, Lewis, R J, Brannigan, J A and Wilkinson, A J (2001) Crystallization and preliminary X-ray analysis of the sporulation factor SpoIIAA in its native and phosphorylated forms. Acta Crystallographica Section D: Biological Crystallography. pp. 292-295. ISSN 0907-4449Full text available as:
Sporulation in Bacillus begins with an asymmetric cell division producing two progeny with identical chromosomes but different developmental fates. As such, it is a simple example of cellular differentiation. The establishment of cell type is controlled by a series of alternate RNA polymerase sigma subunits. The first compartment-specific sigma factor is sigma (F), whose activity is controlled by SpoIIAB, an anti-sigma factor, and SpoIIAA, an anti-sigma factor antagonist which is phosphorylated by the kinase activity of SpoIIAB. Here, the preliminary crystallographic analysis of SpoIIAA and phosphorylated SpoIIAA from B. sphaericus in forms suitable for high-resolution structure determination are reported.
|Copyright, Publisher and Additional Information:||Copyright © 2001 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?ad0136|
|Keywords:||TRANSCRIPTION FACTOR SIGMA(F), BACILLUS-SUBTILIS|
|Academic Units:||The University of York > Chemistry (York)|
|Depositing User:||Sherpa Assistant|
|Date Deposited:||11 May 2005|
|Last Modified:||17 Oct 2013 14:36|