Crystallization and preliminary X-ray analysis of the sporulation factor SpoIIAA in its native and phosphorylated forms

Seavers, P R, Lewis, R J, Brannigan, J A and Wilkinson, A J (2001) Crystallization and preliminary X-ray analysis of the sporulation factor SpoIIAA in its native and phosphorylated forms. Acta Crystallographica Section D: Biological Crystallography. pp. 292-295. ISSN 0907-4449

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Published Version: http://dx.doi.org/10.1107/S090744490001859X

Abstract

Sporulation in Bacillus begins with an asymmetric cell division producing two progeny with identical chromosomes but different developmental fates. As such, it is a simple example of cellular differentiation. The establishment of cell type is controlled by a series of alternate RNA polymerase sigma subunits. The first compartment-specific sigma factor is sigma (F), whose activity is controlled by SpoIIAB, an anti-sigma factor, and SpoIIAA, an anti-sigma factor antagonist which is phosphorylated by the kinase activity of SpoIIAB. Here, the preliminary crystallographic analysis of SpoIIAA and phosphorylated SpoIIAA from B. sphaericus in forms suitable for high-resolution structure determination are reported.

Item Type:	Article
Copyright, Publisher and Additional Information:	Copyright © 2001 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?ad0136
Keywords:	TRANSCRIPTION FACTOR SIGMA(F), BACILLUS-SUBTILIS
Academic Units:	The University of York > Chemistry (York)
Depositing User:	Sherpa Assistant
Date Deposited:	11 May 2005
Last Modified:	19 Feb 2013 12:06
Published Version:	http://dx.doi.org/10.1107/S090744490001859X
Status:	Published
Refereed:	Yes
Related URLs:	http://www.iucr.org/cgi-bin/paper?ad0136
URI:	http://eprints.whiterose.ac.uk/id/eprint/455

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