Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A

Muchova, K, Lewis, R J, Brannigan, J A, Offen, W A, Brown, D P, Barak, I, Youngman, P and Wilkinson, A J (1999) Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A. Acta Crystallographica Section D: Biological Crystallography. pp. 671-676. ISSN 0907-4449

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Published Version: http://dx.doi.org/10.1107/S0907444998012682

Abstract

The key response-regulator gene of sporulation, spo0A, has been cloned from Bacillus stearothermophilus and the encoded protein purified. The DNA-binding and phospho-acceptor domains of Spo0A have been prepared by tryptic digestion of the intact protein and subsequently crystallized in forms suitable for X-ray crystallographic studies. The DNA-binding domain has been crystallized in two forms, one of which diffracts X-rays to beyond 2.5 Angstrom spacing. The crystals of the phospho-acceptor domain diffract X-rays beyond 2.0 Angstrom spacing using synchrotron radiation.

Item Type:	Article
Copyright, Publisher and Additional Information:	Copyright © 1999 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?ad0052
Keywords:	BACILLUS-SUBTILIS SPORULATION, GENE-EXPRESSION, TRANSCRIPTION, BINDING
Academic Units:	The University of York > Chemistry (York)
Depositing User:	Sherpa Assistant
Date Deposited:	11 May 2005
Last Modified:	19 Feb 2013 12:08
Published Version:	http://dx.doi.org/10.1107/S0907444998012682
Status:	Published
Refereed:	Yes
Related URLs:	http://www.iucr.org/cgi-bin/paper?ad0052
URI:	http://eprints.whiterose.ac.uk/id/eprint/454

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