Muchova, K, Lewis, R J, Brannigan, J A, Offen, W A orcid.org/0000-0002-2758-4531, Brown, D P, Barak, I, Youngman, P and Wilkinson, A J orcid.org/0000-0003-4577-9479 (1999) Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A. Acta Crystallographica Section D: Biological Crystallography. pp. 671-676. ISSN 0907-4449
The key response-regulator gene of sporulation, spo0A, has been cloned from Bacillus stearothermophilus and the encoded protein purified. The DNA-binding and phospho-acceptor domains of Spo0A have been prepared by tryptic digestion of the intact protein and subsequently crystallized in forms suitable for X-ray crystallographic studies. The DNA-binding domain has been crystallized in two forms, one of which diffracts X-rays to beyond 2.5 Angstrom spacing. The crystals of the phospho-acceptor domain diffract X-rays beyond 2.0 Angstrom spacing using synchrotron radiation.
|Copyright, Publisher and Additional Information:||Copyright © 1999 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?ad0052|
|Institution:||The University of York|
|Academic Units:||The University of York > Chemistry (York)|
|Depositing User:||Sherpa Assistant|
|Date Deposited:||11 May 2005|
|Last Modified:||20 Apr 2016 15:16|