McVey, C.E., Tolley, S.P., Done, S.H., Brannigan, J.A. and Wilson, K.S. (1997) A new crystal form of penicillin acylase from Escherichia coli. Acta Crystallographica Section D: Biological Crystallography. pp. 777-779. ISSN 0907-4449
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Abstract
A new crystal form of penicillin acylase (penicillin amidohydrolase, E.C. 3.5.1.11) from Escherichia coli W (ATCC 11105) is reported. The crystals were grown using a combination of hanging-drop and streak-seeding methods. The crystals are in the monoclinic space group P2(1) with cell dimensions a = 51.52, b = 131.95, c = 64.43 Angstrom, beta = 106.12 degrees. There is one heterodimer in the asymmetric unit (Vm = 2.45 Angstrom(3) Da(-1)) and the solvent content is 49%. Preliminary data have been collected to d(min) = 2.7 Angstrom using a MAR Research image plate and a rotating-anode X-ray source. Subsequent experiments show diffraction beyond 1.3 Angstrom at a synchrotron radiation source.
| Item Type: | Article |
|---|---|
| Copyright, Publisher and Additional Information: | © 1997 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?jn0030 |
| Keywords: | CRYSTALLIZATION |
| Academic Units: | The University of York > Chemistry (York) |
| Depositing User: | Sherpa Assistant |
| Date Deposited: | 11 May 2005 |
| Last Modified: | 19 Feb 2013 12:08 |
| Published Version: | http://dx.doi.org/10.1107/S0907444997006707 |
| Status: | Published |
| Refereed: | Yes |
| Related URLs: | |
| URI: | http://eprints.whiterose.ac.uk/id/eprint/450 |
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