White Rose University Consortium logo
University of Leeds logo University of Sheffield logo York University logo

Crystallization and preliminary crystallographic analysis of the DNA gyrase B protein from B-stearothermophilus

Tsai, F T F, Subramanya, H S, Brannigan, J A, Wilkinson, A J and Wigley, D B (1996) Crystallization and preliminary crystallographic analysis of the DNA gyrase B protein from B-stearothermophilus. Acta Crystallographica Section D: Biological Crystallography. pp. 1216-1218. ISSN 0907-4449

Full text available as:
[img]
Preview
Text (branniganja7.pdf)
branniganja7.pdf

Download (775Kb)

Abstract

DNA gyrase B (GyrB) from B. stearothermophilus has been crystallized in the presence of the non-hydrolyzable ATP analogue, 5'-adenylpl-beta-gamma-imidodiphosphate (ADPNP), by the dialysis method. A complete native data set to 3.7 Angstrom has been collected from crystals which belonged to the cubic space group I23 with unit-cell dimension a = 250.6 Angstrom. Self-rotation function analysis indicates the position of a molecular twofold axis. Low-resolution data sets of a thimerosal and a selenomethionine derivative have also been analysed. The heavy-atom positions are consistent with one dimer in the asymmetric unit.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 1996 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?gr0637
Institution: The University of York
Academic Units: The University of York > Chemistry (York)
Depositing User: Sherpa Assistant
Date Deposited: 11 May 2005
Last Modified: 16 Oct 2014 23:31
Published Version: http://dx.doi.org/10.1107/S0907444996008979
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/449

Actions (repository staff only: login required)