Tsai, F T F, Subramanya, H S, Brannigan, J A, Wilkinson, A J and Wigley, D B (1996) Crystallization and preliminary crystallographic analysis of the DNA gyrase B protein from B-stearothermophilus. Acta Crystallographica Section D: Biological Crystallography. pp. 1216-1218. ISSN 0907-4449Full text available as:
DNA gyrase B (GyrB) from B. stearothermophilus has been crystallized in the presence of the non-hydrolyzable ATP analogue, 5'-adenylpl-beta-gamma-imidodiphosphate (ADPNP), by the dialysis method. A complete native data set to 3.7 Angstrom has been collected from crystals which belonged to the cubic space group I23 with unit-cell dimension a = 250.6 Angstrom. Self-rotation function analysis indicates the position of a molecular twofold axis. Low-resolution data sets of a thimerosal and a selenomethionine derivative have also been analysed. The heavy-atom positions are consistent with one dimer in the asymmetric unit.
|Copyright, Publisher and Additional Information:||Copyright © 1996 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?gr0637|
|Institution:||The University of York|
|Academic Units:||The University of York > Chemistry (York)|
|Depositing User:||Sherpa Assistant|
|Date Deposited:||11 May 2005|
|Last Modified:||26 Mar 2015 07:37|