Goode, NP, Davison, AM, Gowland, G and Shires, M (1986) Preparation, purification and analysis of BSA-coated latex particles for invivo studies. Non-ionic adsorbance of unbound protein ligand. Journal of Immunological Methods, 92 (1). 31 - 35 . ISSN 0022-1759
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Published Version: http://dx.doi.org/10.1016/0022-1759(86)90500-4
Abstract
A procedure for the preparation, purification and analysis of small, BSA-coated carboxylate-modified latex (CML) particles, suitable for use in in vivo studies has been developed. Following conjugation, uptake of unbound BSA by Amberlite XAD-8 non-ionic adsorbent beads has been shown to be an effective method by which unbound protein ligands may be removed from coated latex preparations.
| Item Type: | Article |
|---|---|
| Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) |
| Depositing User: | Symplectic Publications |
| Date Deposited: | 23 Apr 2012 09:33 |
| Last Modified: | 08 Feb 2013 17:37 |
| Published Version: | http://dx.doi.org/10.1016/0022-1759(86)90500-4 |
| Status: | Published |
| Publisher: | Elsevier |
| Identification Number: | 10.1016/0022-1759(86)90500-4 |
| URI: | http://eprints.whiterose.ac.uk/id/eprint/43873 |
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