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Cationic colloidal gold--a novel marker for the demonstration of glomerular polyanion status in routine renal biopsies

Goode, NP, Shires, M, Aparicio, SR and Davison, AM (1991) Cationic colloidal gold--a novel marker for the demonstration of glomerular polyanion status in routine renal biopsies. Nephrology Dialysis Transplantation, 6 (12). 923 - 930 . ISSN 0931-0509

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Abstract

Investigations of glomerular anionic charge status in human renal biopsies have previously been restricted, by the techniques and markers used, to staining of sites in pre-embedded tissue. The introduction of a novel marker, cationic colloidal gold, which demonstrates fixed anionic sites in hydrophilic resin (LR Gold)-embedded, ultrathin tissue sections, has now enabled glomerular charge to be evaluated in routine biopsy material. The cationic gold marker detects components which express anionic charge under different pH conditions. The patterns of staining in tissue showing minor glomerular pathology and low proteinuria, together with enzyme-digestion studies indicate that anionic sites are normally associated with heparan sulphate proteoglycans, glycocalyx sialoproteins, hyaluronic acid, and other GBM components which have not yet been characterised. Several charge aberrations involving different pathological mechanisms have been identified using cationic gold. These aberrations may be categorised according to the pathological basis of the charge pattern defect, rather than glomerular disease classification, as a prelude to the precise identification of the anionic sites and their functional importance in relation to the glomerular charge selectivity barrier. The categories which have been defined are: (1) 'Normal', (2) interrupted, (3) neutralised, (4) structurally disorganised, and (5) depleted. As sites are further characterised sub-categorisation is likely. We anticipate that this approach will help to elucidate both the participation of charged components in disease pathogenesis and their role in relation to glomerular proteinuria.

Item Type: Article
Keywords: adolescent, aged, anions, basement membrane, binding sites, biological markers, child, preschool, female, gold, humans, kidney diseases, kidney glomerulus, male, microscopy, electron, middle aged, staining and labeling
Institution: The University of Leeds
Academic Units: The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds)
Depositing User: Symplectic Publications
Date Deposited: 16 May 2012 11:44
Last Modified: 08 Feb 2013 17:37
Status: Published
Publisher: Oxford University Press
Identification Number: 10.1093/ndt/6.12.923
URI: http://eprints.whiterose.ac.uk/id/eprint/43870

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