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Genetic manipulation of stomatal density influences stomatal size, plant growth and tolerance to restricted water supply across a growth carbon dioxide gradient

Doheny-Adams, T., Hunt, L., Franks, P. J., Beerling, D. J. and Gray, J. E. (2012) Genetic manipulation of stomatal density influences stomatal size, plant growth and tolerance to restricted water supply across a growth carbon dioxide gradient. Philosophical Transactions of the Royal Society B: Biological Sciences, 367 (1588). p. 547. ISSN 0962-8436

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Abstract

To investigate the impact of manipulating stomatal density, a collection of Arabidopsis epidermal patterning factor (EPF) mutants with an approximately 16-fold range of stomatal densities (approx. 20-325% of that of control plants) were grown at three atmospheric carbon dioxide (CO(2)) concentrations (200, 450 and 1000 ppm), and 30 per cent or 70 per cent soil water content. A strong negative correlation between stomatal size (S) and stomatal density (D) was observed, suggesting that factors that control D also affect S. Under some but not all conditions, mutant plants exhibited abnormal stomatal density responses to CO(2) concentration, suggesting that the EPF signalling pathway may play a role in the environmental adjustment of D. In response to reduced water availability, maximal stomatal conductance was adjusted through reductions in S, rather than D. Plant size negatively correlated with D. For example, at 450 ppm CO(2) EPF2-overexpressing plants, with reduced D, had larger leaves and increased dry weight in comparison with controls. The growth of these plants was also less adversely affected by reduced water availability than plants with higher D, indicating that plants with low D may be well suited to growth under predicted future atmospheric CO(2) environments and/or water-scarce environments.

Item Type: Article
Copyright, Publisher and Additional Information: © 2012 The Royal Society. This is an author produced version of a paper subsequently published in Philosophical Transactions of the Royal Society B: Biological Sciences. Uploaded in accordance with the publisher's self-archiving policy.
Keywords: stomatal density (D); stomatal size (S); carbon dioxide; signalling peptide; plant growth; epidermal patterning factor
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield)
The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield)
Depositing User: Miss Anthea Tucker
Date Deposited: 09 Mar 2012 13:46
Last Modified: 08 Feb 2013 17:37
Published Version: http://dx.doi.org/10.1098/rstb.2011.0272
Status: Published
Publisher: Royal Society
Identification Number: 10.1098/rstb.2011.0272
URI: http://eprints.whiterose.ac.uk/id/eprint/43758

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