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Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells

Green, L.R., Monk, P.N., Partridge, L.J., Morris, P., Gorringe, A.R. and Read, R.C. (2011) Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells. Infection and Immunity, 79 (6). pp. 2241-2249. ISSN 0019-9567

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Abstract

The tetraspanins are a superfamily of transmembrane proteins with diverse functions and can form extended microdomains within the plasma membrane in conjunction with partner proteins, which probably includes receptors for bacterial adhesins. Neisseria meningitidis, the causative agent of meningococcal disease, attaches to host nasopharyngeal epithelial cells via type IV pili and opacity (Opa) proteins. We examined the role of tetraspanin function in Neisseria meningitidis adherence to epithelial cells. Tetraspanins CD9, CD63, and CD151 were expressed by HEC-1-B and DETROIT 562 cells. Coincubation of cells with antibodies against all three tetraspanin molecules used individually or in combination, with recombinant tetraspanin extracellular domains (EC2), or with small interfering RNAs (siRNAs) significantly reduced adherence of Neisseria meningitidis. In contrast, recombinant CD81, a different tetraspanin, had no effect on meningococcal adherence. Antitetraspanin antibodies reduced the adherence to epithelial cells of Neisseria meningitidis strain derivatives expressing Opa and pili significantly more than isogenic strains lacking these determinants. Adherence to epithelial cells of strains of Staphylococcus aureus, Neisseria lactamica, Escherichia coli, and Streptococcus pneumoniae was also reduced by pretreatment of cells with tetraspanin antibodies and recombinant proteins. These data suggest that tetraspanins are required for optimal function of epithelial adhesion platforms containing specific receptors for Neisseria meningitidis and potentially for multiple species of bacteria.

Item Type: Article
Copyright, Publisher and Additional Information: © 2011 American Society for Microbiology. This is an author produced version of a paper subsequently published in Infection and Immunity. Uploaded in accordance with the publisher's self-archiving policy.
Keywords: Human Endothelial-Cells; Pathogenic Neisseriae; Pilus Receptor; Uroplakin-IA; OPA Proteins; Binding; Membrane; Antigen; Fusion; CD81
Academic Units: The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine (Sheffield) > Department of Infection & Immunity
Depositing User: Miss Anthea Tucker
Date Deposited: 04 Jul 2011 08:20
Last Modified: 08 Feb 2013 17:32
Published Version: http://dx.doi.org/10.1128/IAI.01354-10
Status: Published
Publisher: American Society for Microbiology
Refereed: Yes
Identification Number: 10.1128/IAI.01354-10
URI: http://eprints.whiterose.ac.uk/id/eprint/43101

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