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Regulation of zebrafish hatching by tetraspanin cd63

Trikic, M.Z., Monk, P., Roehl, H. and Partridge, L.J. (2011) Regulation of zebrafish hatching by tetraspanin cd63. Plos One , 6 (5). Art no. e19858. ISSN 1932-6203

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Abstract

Tetraspanins cause the clustering of membrane proteins into a level of organisation essential for cellular function. Given the importance and complicated nature of this mechanism, we attempted a novel approach to identify the function of a single component in a biologically relevant context. A morpholino knockdown strategy was used to investigate the role of cd63, a membrane protein associated with intracellular transport and a melanoma marker, in embryonic zebrafish. By using three separate morpholinos targeting cd63, we were able to identify a specific phenotype. Strikingly, morphant fish failed to hatch due to the lack of secreted proteolytic enzymes required for chorion-softening. The morphology of the hatching gland at both the cellular and intracellular levels was disorganised, suggesting a role for cd63 in the functioning of this organ. This work identifies a specific role for cd63 in the zebrafish embryo and provides evidence for the suitability of zebrafish as a model system for the investigation of tetraspanin enriched microdomains.

Item Type: Article
Copyright, Publisher and Additional Information: © 2011 Trikić et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Cathepsin-L; Secretory Lysosomes; Gene-Expression; Dendritic Cells; Protein CD63; Wild-Type; Differentiation; Membrane; Vesicles; Biosynthesis
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine (Sheffield) > Department of Infection & Immunity
The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Biomedical Science (Sheffield)
Depositing User: Miss Anthea Tucker
Date Deposited: 21 Jun 2011 08:17
Last Modified: 18 Jun 2014 14:18
Published Version: http://dx.doi.org/10.1371/journal.pone.0019683
Status: Published
Publisher: Public Library Science
Refereed: Yes
Identification Number: 10.1371/journal.pone.0019683
URI: http://eprints.whiterose.ac.uk/id/eprint/43075

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