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Evaluation of tissue engineered models of the oral mucosa to investigate oral candidiasis

Yadev, N.R., Murdoch, C., Saville, S.P. and Thornhill, M.H. (2011) Evaluation of tissue engineered models of the oral mucosa to investigate oral candidiasis. Microbial Pathogenesis, 50 (6). pp. 278-285. ISSN 0882-4010


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Candida albicans is a commensal organism that can be isolated from the majority of healthy individuals. However, in certain susceptible individuals C. albicans can become pathogenic leading to the mucocutaneous infection; oral candidiasis. Murine models and in vitro monolayer cultures have generated some data on the likely virulence and host factors that contribute to oral candidiasis but these models have limitations. Recently, tissue engineered oral mucosal models have been developed to mimic the normal oral mucosa but little information is available on their true representation. In this study, we assessed the histological features of three different tissue engineered oral mucosal models compared to the normal oral mucosa and analysed both cell damage and cytokine release following infection with C albicans. Models comprised of normal oral keratinocytes and a fibroblast-containing matrix displayed more similar immunohistological and proliferation characteristics to normal mucosa, compared to models composed of an oral carcinoma cell line. Although all models were invaded and damaged by C. albicans in a similar manner, the cytokine response was much more pronounced in models containing normal keratinocytes. These data suggest that models based on normal keratinocytes atop a fibroblast-containing connective tissue will significantly aid in dissecting the molecular pathogenesis of oral candidiasis. (C) 2011 Elsevier Ltd. All rights reserved.

Item Type: Article
Copyright, Publisher and Additional Information: © 2011 Elsevier. This is an author produced version of a paper subsequently published in Microbial Pathogenesis. Uploaded in accordance with the publisher's self-archiving policy.
Keywords: Candida albicans; Tissue engineering; Oral mucosa; Oral candidiasis
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Dentistry (Sheffield)
Depositing User: Miss Anthea Tucker
Date Deposited: 21 Jun 2011 08:16
Last Modified: 08 Feb 2013 17:32
Published Version: http://dx.doi.org/10.1016/j.micpath.2010.11.009
Status: Published
Publisher: Elsevier
Refereed: Yes
Identification Number: 10.1016/j.micpath.2010.11.009
URI: http://eprints.whiterose.ac.uk/id/eprint/43072

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