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A proteomic and phosphoproteomic analysis of Oryza sativa plasma membrane and vacuolar membrane

Whiteman, S.A., Nuhse, T.S., Ashford, D.A., Sanders, D. and Maathuis, F.J.M. (2008) A proteomic and phosphoproteomic analysis of Oryza sativa plasma membrane and vacuolar membrane. Plant Journal. pp. 146-156. ISSN 0960-7412

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Abstract

Proteomic and phosphoproteomic analyses of rice shoot and root tonoplast-enriched and plasma membrane-enriched membrane fractions were carried out to look at tissue-specific expression, and to identify putative regulatory sites of membrane transport proteins. Around 90 unique membrane proteins were identified, which included primary and secondary transporters, ion channels and aquaporins. Primary H+ pumps from the AHA family showed little isoform specificity in their tissue expression pattern, whereas specific isoforms of the Ca2+ pump ECA/ACA family were expressed in root and shoot tissues. Several ABC transporters were detected, particularly from the MDR and PDR subfamilies, which often showed expression in either roots or shoots. Ammonium transporters were expressed in root, but not shoot, tissue. Large numbers of sugar transporters were expressed, particularly in green tissue. The occurrence of phosphorylation sites in rice transporters such as AMT1;1 and PIP2;6 agrees with those previously described in other species, pointing to conserved regulatory mechanisms. New phosphosites were found in many transporters, including H+ pumps and H+:cation antiporters, often at residues that are well conserved across gene families. Comparison of root and shoot tissue showed that phosphorylation of AMT1;1 and several further transporters may be tissue dependent.

Item Type: Article
Copyright, Publisher and Additional Information: © 2008 The Authors. This is an author produced version of a paper published in Plant Journal. Uploaded in accordance with the publisher's self archiving policy.
Keywords: proteomics, phosphoproteomics, membrane transport, Oryza sativa, rice, ARABIDOPSIS-THALIANA, ABC TRANSPORTER, H+-ATPASE, K+ UPTAKE, PLANT, RICE, PROTEINS, IDENTIFICATION, EXPRESSION, REVEALS, Plant Science, Cell Biology, Genetics
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: Repository Administrator York
Date Deposited: 19 Nov 2008 15:45
Last Modified: 09 Jul 2014 11:05
Published Version: http://dx.doi.org/10.1111/j.1365-313X.2008.03578.x
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/4153

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