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Co-existing grass species have distinctive arbuscular mycorrhizal communities

Vandenkoornhuyse, P, Ridgway, K P, Watson, I J, Fitter, A H and Young, J P W (2003) Co-existing grass species have distinctive arbuscular mycorrhizal communities. Molecular Ecology. pp. 3085-3095. ISSN 0962-1083

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Abstract

Arbuscular mycorrhizal (AM) fungi are biotrophic symbionts colonizing the majority of land plants, and are of major importance in plant nutrient supply. Their diversity is suggested to be an important determinant of plant community structure, but the influence of host-plant and environmental factors on AM fungal community in plant roots is poorly documented. Using the terminal restriction fragment length polymorphism (T-RFLP) strategy, the diversity of AM fungi was assessed in 89 roots of three grass species (Agrostis capillaris, Festuca rubra, Poa pratensis) that co-occurred in the same plots of a field experiment. The impact of different soil amendments (nitrogen, lime, nitrogen and lime) and insecticide application on AM fungal community was also studied. The level of diversity found in AM fungal communities using the T-RFLP strategy was consistent with previous studies based on clone libraries. Our results clearly confirm that an AM fungal host-plant preference exists, even between different grass species. AM communities colonizing A. capillaris were statistically different from the others (P < 0.05). Although grass species evenness changed in amended soils, AM fungal community composition in roots of a given grass species remained stable. Conversely, in plots where insecticide was applied, we found higher AM fungal diversity and, in F. rubra roots, a statistically different AM fungal community.

Item Type: Article
Copyright, Publisher and Additional Information: © 2003 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=0962-1083 or www.blackwell-synergy.com
Keywords: AM fungi, host-plant preference, soil treatment effect, SSU rRNA gene, T-RFLP, trnL intron, 16S RIBOSOMAL-RNA, LENGTH-POLYMORPHISM ANALYSIS, BACTERIAL COMMUNITIES, MOLECULAR-IDENTIFICATION, FUNGAL DIVERSITY, MARINE-SEDIMENTS, GLOMUS-MOSSEAE, PLANT-ROOTS, SOIL, PHYLOGENY
Academic Units: The University of York > Biology (York)
Depositing User: Peter Young
Date Deposited: 22 Apr 2005
Last Modified: 17 Oct 2013 14:28
Published Version: http://dx.doi.org/10.1046/j.1365-294X.2003.01967.x
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/414

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