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Molecular diversity of arbuscular mycorrhizal fungi and patterns of host association over time and space in a tropical forest

Husband, R, Herre, E.A., Gallery, R., Young, J.P.W. and Turner, S.L. (2002) Molecular diversity of arbuscular mycorrhizal fungi and patterns of host association over time and space in a tropical forest. Molecular Ecology. pp. 2669-2678. ISSN 0962-1083

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Abstract

We have used molecular techniques to investigate the diversity and distribution of the arbuscular mycorrhizal (AM) fungi colonizing tree seedling roots in the tropical forest on Barro Colorado Island (BCI), Republic of Panama. In the first year, we sampled newly emergent seedlings of the understory treelet Faramea occidentalis and the canopy emergent Tetragastris panamensis, from mixed seedling carpets at each of two sites. The following year we sampled surviving seedlings from these cohorts. The roots of 48 plants were analysed using AM fungal-specific primers to amplify and clone partial small subunit (SSU) ribosomal RNA gene sequences. Over 1300 clones were screened for random fragment length polymorphism (RFLP) variation and 7% of these were sequenced. Compared with AM fungal communities sampled from temperate habitats using the same method, the overall diversity was high, with a total of 30 AM fungal types identified. Seventeen of these types have not been recorded previously, with the remainder being similar to types reported from temperate habitats. The tropical mycorrhizal population showed significant spatial heterogeneity and nonrandom associations with the different hosts. Moreover there was a strong shift in the mycorrhizal communities over time. AM fungal types that were dominant in the newly germinated seedlings were almost entirely replaced by previously rare types in the surviving seedlings the following year. The high diversity and huge variation detected across time points, sites and hosts, implies that the AM fungal types are ecologically distinct and thus may have the potential to influence recruitment and host composition in tropical forests.

Item Type: Article
Copyright, Publisher and Additional Information: © 2002 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=0962-1083 or www.blackwell-synergy.com
Keywords: AM1, arbuscular mycorrhiza, community composition, diversity, SSU, tropical forest, PLANT COMMUNITY STRUCTURE, TALLGRASS PRAIRIE, GLOMUS-MOSSEAE, GROWTH, SPORES, SUCCESSION, GRASSLAND, GLOMALES, COLONIZATION, PRODUCTIVITY
Academic Units: The University of York > Biology (York)
Depositing User: Peter Young
Date Deposited: 22 Apr 2005
Last Modified: 17 Oct 2013 14:31
Published Version: http://dx.doi.org/10.1046/j.1365-294X.2002.01647.x
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/413

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