Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Ponchel, F., Toomes, C., Bransfield, K., Leong, F.T., Douglas, S.H., Field, S.L., Bell, S.M., Combaret, V., Puisieux, A., Mighell, A.J., Robinson, P.A., Inglehearn, C.F., Isaacs, J.D. and Markham, A.F. (2003) Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions. BMC Biotechnology, 3 (18). ISSN 1472-6750

Abstract

Metadata

Authors/Creators:
  • Ponchel, F.
  • Toomes, C.
  • Bransfield, K.
  • Leong, F.T.
  • Douglas, S.H.
  • Field, S.L.
  • Bell, S.M.
  • Combaret, V.
  • Puisieux, A.
  • Mighell, A.J.
  • Robinson, P.A.
  • Inglehearn, C.F.
  • Isaacs, J.D.
  • Markham, A.F.
Copyright, Publisher and Additional Information: © 2003 Ponchel et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
Keywords: Real-time PCR, SYBR-green, rearrangement, amplification, deletion
Institution: The University of Leeds
Academic Units: The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Genetics (Leeds)
Depositing User: Repository Officer
Date Deposited: 13 Mar 2006
Last Modified: 18 Sep 2016 13:36
Published Version: http://www.biomedcentral.com/1472-6750/3/18
Status: Published
Refereed: Yes
Identification Number: 10.1186/1472-6750-3-18

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Filename: 1472-6750-3-18.pdf

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