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Cloning, sequencing, and characterization of the hexahydro-1,3,5-trinitro-1,3,5-triazine degradation gene cluster from Rhodococcus rhodochrous

Seth-Smith, H M B, Rosser, S J, Basran, A, Travis, E R, Dabbs, E R, Nicklin, S and Bruce, N C (2002) Cloning, sequencing, and characterization of the hexahydro-1,3,5-trinitro-1,3,5-triazine degradation gene cluster from Rhodococcus rhodochrous. Applied and Environmental Microbiology. pp. 4764-4771. ISSN 0099-2240

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Abstract

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a high explosive which presents an environmental hazard as a major land and groundwater contaminant. Rhodococcus rhodochrous strain 11Y was isolated from explosive contaminated land and is capable of degrading RDX when provided as the sole source of nitrogen for growth. Products of RDX degradation in resting-cell incubations were analyzed and found to include nitrite, formaldehyde, and formate. No ammonium was excreted into the medium, and no dead-end metabolites were observed. The gene responsible for the degradation of RDX in strain 11Y is a constitutively expressed cytochrome P450-like gene, xpLA, which is found in a gene cluster with an adrenodoxin reductase homologue, xplB. The cytochrome P450 also has a flavodoxin domain at the N terminus. This study is the first to present a gene which has been identified as being responsible for RDX biodegradation. The mechanism of action of XplA on RDX is thought to involve initial denitration followed by spontaneous ring cleavage and mineralization.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2002 American Society for Microbiology
Keywords: HERBICIDE-INDUCIBLE CYTOCHROMES-P-450, RDX HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE, PHANEROCHAETE-CHRYSOSPORIUM, TNT 2,4,6-TRINITROTOLUENE, STREPTOMYCES-GRISEOLUS, ADRENODOXIN REDUCTASE, BIODEGRADATION, FLAVODOXIN, OPERON, STRAIN
Institution: The University of York
Academic Units: The University of York > Biology (York) > Centre for Novel Agricultural Products (CNAP) (York)
Depositing User: Sherpa Assistant
Date Deposited: 01 Apr 2005
Last Modified: 14 Oct 2014 14:43
Published Version: http://dx.doi.org/10.1128/AEM.68.10.4764-4771.2002
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/375

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