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Isolation and characterization of microsatellite loci from two inbreeding bark beetle species (Coccotrypes)

Berg, P.R., Dawson, D.A., Pandhal, J., Kirkendall, L.R. and Burke, T. (2003) Isolation and characterization of microsatellite loci from two inbreeding bark beetle species (Coccotrypes). Molecular Ecology Notes, 3 (2). pp. 270-273. ISSN 1471-8278

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Abstract

We developed 14 microsatellite markers in Coccotrypes carpophagus and 14 in C. dactyliperda. These loci will be used for studying genetic structure and the level of inbreeding in populations in the Canary Islands and Madeira. As a result of long-term inbreeding, genetic variability is relatively low in these bark beetle species. We found one to five alleles per locus in 29 C. carpophagus and 41 C. dactyliperda from various localities. Eleven of the markers developed for C. carpophagus amplified in C. dactyliperda and seven of the markers developed for C. dactyliperda amplified in C. carpophagus.

Item Type: Article
Copyright, Publisher and Additional Information: © 2003 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology Notes: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=1471-8278 or http://www.blackwell-synergy.com/
Keywords: bark beetle, Coccotrypes, cross-species amplification, genetic population structure, inbreeding, microsatellite
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield)
Depositing User: Sherpa Assistant
Date Deposited: 31 Mar 2005
Last Modified: 04 Jun 2014 09:17
Published Version: http://www.blackwell-synergy.com/
Status: Published
Refereed: Yes
Identification Number: 10.1046/j.1471-8286.2003.00423.x
URI: http://eprints.whiterose.ac.uk/id/eprint/370

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