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A critique of avian CHD-based molecular sexing protocols illustrated by a Z-chromosome polymorphism detected in auklets

Dawson, D.A., Darby, S., Hunter, F.M., Krupa, A.P., Jones, I.L. and Burke, T. (2001) A critique of avian CHD-based molecular sexing protocols illustrated by a Z-chromosome polymorphism detected in auklets. Molecular Ecology Notes, 1 (3). pp. 201-204. ISSN 1471-8278

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The sexes of non-ratite birds can be determined routinely by PCR amplification of the CHD-Z and CHD-W genes. CHD -based molecular sexing of four species of auklets revealed the presence of a polymorphism in the Z chromosome. No deviation from a 1:1 sex ratio was observed among the chicks, though the analyses were of limited power. Polymorphism in the CHD-Z gene has not been reported previously in any bird, but if undetected it could lead to the incorrect assignment of sex. We discuss the potential difficulties caused by a polymorphism such as that identified in auklets and the merits of alternative CHD -based sexing protocols and primers.

Item Type: Article
Copyright, Publisher and Additional Information: © 2001 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology Notes: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=1471-8278 or http://www.blackwell-synergy.com/
Keywords: Aethia, Alcidae, auklet, CHD genes, Laridae, molecular sex determination
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield)
Depositing User: Sherpa Assistant
Date Deposited: 31 Mar 2005
Last Modified: 04 Jun 2014 09:31
Published Version: http://www.blackwell-synergy.com/
Status: Published
Refereed: Yes
Identification Number: 10.1046/j.1471-8278.2001.00060.x
URI: http://eprints.whiterose.ac.uk/id/eprint/364

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