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Decontamination of orthodontic bands following size determination and cleaning

Benson, P.E. and Douglas, C.W. (2007) Decontamination of orthodontic bands following size determination and cleaning. Journal of Orthodontics, 34 (1). pp. 18-24. ISSN 1465-3133

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Abstract

OBJECTIVE: To measure the effectiveness of ultrasonic cleaning for decontaminating orthodontic molar bands following size determination using a quantitative antibody capture assay technique.

DESSIGN: A prospective, cross-sectional, clinical and laboratory investigation.

SETTING: The Orthodontic Department of the Charles Clifford Dental Hospital and the Microbiology Laboratory of the School of Clinical Dentistry, Sheffield

PARTICIPANTS: Thirty-two patients about to start orthodontic treatment with fixed orthodontic appliances.

METHODS: Four first molar bands were tried in the mouth and then removed. They were randomly assigned either for no decontamination (control) or to be decontaminated in an ultrasonic cleaning bath for 15 minutes (experimental). The bands were placed in a predetermined volume of phosphate-buffered saline (PBS) and assayed by enzyme-linked immunosorbent assay (ELISA) for albumin, to detect the presence of blood and amylase, to detect the presence of saliva.

RESULTS: Fifty per cent of decontaminated molar bands showed detectable amounts of amylase, albumin or both. The quantity of detectable amylase was significantly reduced on the cleaned compared with uncleaned bands (P = 0.036); however, the reduction in the quantity of albumin was not statistically significant (P = 0.074).

CONCLUSIONS: Ultrasonic cleaning for 15 minutes reduces, but does not always eliminate, salivary proteins (amylase) from tried-in bands. It is less effective at removing serum protein (albumin). There is a need, therefore, to investigate effective means of cleaning organic material from orthodontic bands if they are to be adequately sterilized and reused.

Item Type: Article
Academic Units: The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Dentistry (Sheffield) > Department of Oral Health and Development (Sheffield)
Depositing User: Sherpa Assistant
Date Deposited: 31 Oct 2008 11:45
Last Modified: 08 Feb 2013 16:57
Published Version: http://dx.doi.org/10.1179/146531207225021879
Status: Published
Publisher: Maney
Refereed: Yes
Identification Number: 10.1179/146531207225021879
URI: http://eprints.whiterose.ac.uk/id/eprint/3639

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