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Diversity of bacteria associated with natural aphid populations

Haynes, S., Daniell, T.J., Webster, G., van Veen, F.J.F., Godfray, H.C.J., Darby, A.C., Prosser, J.I. and Douglas, A.E. (2003) Diversity of bacteria associated with natural aphid populations. Applied and Environmental Microbiology. pp. 7216-7223. ISSN 0099-2240

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Abstract

The bacterial communities of aphids were investigated by terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis analysis of 16S rRNA gene fragments generated by PCR with general eubacterial primers. By both methods, the -proteobacterium Buchnera was detected in laboratory cultures of six parthenogenetic lines of the pea aphid Acyrthosiphon pisum and one line of the black bean aphid Aphis fabae, and one or more of four previously described bacterial taxa were also detected in all aphid lines except one of A. pisum. These latter bacteria, collectively known as secondary symbionts or accessory bacteria, comprised three taxa of -proteobacteria (R-type [PASS], T-type [PABS], and U-type [PAUS]) and a rickettsia (S-type [PAR]). Complementary analysis of aphids from natural populations of four aphid species (A. pisum [n 74], Amphorophora rubi [n 109], Aphis sarothamni [n 42], and Microlophium carnosum [n 101]) from a single geographical location revealed Buchnera and up to three taxa of accessory bacteria, but no other bacterial taxa, in each aphid. The prevalence of accessory bacterial taxa varied significantly among aphid species but not with the sampling month (between June and August 2000). These results indicate that the accessory bacterial taxa are distributed across multiple aphid species, although with variable prevalence, and that laboratory culture does not generally result in a shift in the bacterial community in aphids. Both the transmission patterns of the accessory bacteria between individual aphids and their impact on aphid fitness are suggested to influence the prevalence of accessory bacterial taxa in natural aphid populations.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2003 American Society for Microbiology
Academic Units: The University of York > Biology (York)
Depositing User: Sherpa Assistant
Date Deposited: 04 Mar 2005
Last Modified: 17 Oct 2013 14:31
Published Version: http://dx.doi.org/10.1128/AEM.69.12.7336-7342.2003
Status: Published
Refereed: Yes
URI: http://eprints.whiterose.ac.uk/id/eprint/329

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