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alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis

Kumar, A., Brannigan, J.A. and Moran, C.P. (2004) alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis. Journal of Bacteriology. pp. 1078-1083. ISSN 0021-9193

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Abstract

At the onset of endospore formation in Bacillus subtilis, the DNA binding protein Spo0A activates transcription from two types of promoters. The first type includes the spoIIG and spoIIE promoters, which are used by sigma(A)-RNA polymerase, whereas the second type includes the spoIIA promoter, which is used by RNA polymerase containing the secondary sigma factor sigma(H). Previous genetic analyses have identified specific amino acids in alpha-helix E of Spo0A that are important for activation of Spo0A-dependent, sigma(A)-dependent promoters. However, these amino acids are not required for activation of the sigma(H)-dependent spoIIA promoter. We now report the effects of additional single-amino-acid substitutions and the effects of deletions in alpha-helix E. The effects of alanine substitutions revealed one new position (239) in Spo0A that appears to be specifically required for activation of the sigma(A)-dependent promoters. Based on the effects of a deletion mutation, we suggest that alpha-helix E in Spo0A is not directly involved in interaction with sigma(H)-RNA polymerase.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2004 American Society for Microbiology
Keywords: RESPONSE REGULATOR, TRANSCRIPTION FACTOR, SPORULATION, PHOSPHORELAY, INITIATION, BINDING, REGION
Academic Units: The University of York > Chemistry (York)
Depositing User: Sherpa Assistant
Date Deposited: 04 Mar 2005
Last Modified: 17 Oct 2013 14:28
Published Version: http://dx.doi.org/10.1128/JB.186.4.1078-1083.2004
Status: Published
Refereed: Yes
URI: http://eprints.whiterose.ac.uk/id/eprint/311

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