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Higher diversity of Rhizobium leguminosarum biovar viciae populations in arable soils than in grass soils

Palmer, K M and Young, J P W (2000) Higher diversity of Rhizobium leguminosarum biovar viciae populations in arable soils than in grass soils. Applied and Environmental Microbiology. pp. 2445-2450. ISSN 0099-2240

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Abstract

The bacterial genetic diversity after long-term arable cultivation was compared with that under permanent grassland using replicated paired contrasts, Pea-nodulating Rhizobium leguminosarum populations were sampled from pairs of arable and grass sites at four locations in Yorkshire, United Kingdom, isolates were characterized using both chromosomal (16S-23S ribosomal DNA internal transcribed spacer PCR-restriction fragment length polymorphism) and plasmid (group-specific repC PCR amplification) markers. The diversities of chromosomal types, repC profiles, and combined genotypes were calculated using richness in types (adjusted to equal sample sizes by rarefaction), Shannon-Wiener index, and Simpson's index. The relative differences in diversity within each pair of sites were similar for all three diversity measures, Chromosomal types, repC profiles, and combined genotypes were each more diverse in arable soils than in grass soils at two of the four locations. The other comparisons showed no significant differences. We conclude that rhizobial diversity can be affected by differences between these two management regimens. Multiple regression analyses indicated that lower diversity was associated with high potential nitrogen and phosphate levels or with acidity.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2000 American Society for Microbiology
Keywords: PLASMID-REPLICATION SEQUENCES, GENETIC DIVERSITY, BV-TRIFOLII, ENZYME POLYMORPHISM, FIELD POPULATIONS
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: Peter Young
Date Deposited: 04 Mar 2005
Last Modified: 15 Oct 2014 04:15
Status: Published
Refereed: Yes
URI: http://eprints.whiterose.ac.uk/id/eprint/303

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