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The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity

Guo, X., Yang, H., Debelle, F., Yang, G., Denarie, J., Young, J.P.W., Li, F., Zhang, X. and Turner, S.L. (2000) The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity. Applied and Environmental Microbiology. pp. 2988-2995. ISSN 0099-2240

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Abstract

The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2000 American Society for Microbiology
Keywords: NOD FACTORS, NUCLEOTIDE-SEQUENCE, FATTY-ACIDS, MELILOTI, HUAKUII, LEGUMINOSARUM, PROTEINS, IDENTIFICATION, BIOSYNTHESIS, ACTIVATION
Institution: The University of York
Academic Units: The University of York > Biology (York)
The University of York > English and Related Literature (York)
Depositing User: Peter Young
Date Deposited: 04 Mar 2005
Last Modified: 14 Oct 2014 18:13
Status: Published
Refereed: Yes
URI: http://eprints.whiterose.ac.uk/id/eprint/296

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