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Symbiotic and genetic diversity of Rhizobium galegae isolates collected from the Galega orientalis gene center in the Caucasus

Andronov, E.E., Roumiantseva, M.L., Dzyubenko, N.I., Onichtchouk, O.P., Kurchak, O.N., Dresler-Nurmi, A., Young, J.P.W., Simarov, B.V., Terefework, Z. and Lindstrom, K. (2003) Symbiotic and genetic diversity of Rhizobium galegae isolates collected from the Galega orientalis gene center in the Caucasus. Applied and Environmental Microbiology. pp. 1067-1074. ISSN 0099-2240

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Abstract

This paper explores the relationship between the genetic diversity of rhizobia and the morphological diversity of their plant hosts. Rhizobium galegae strains were isolated from nodules of wild Galega orientalis and Galega officinalis in the Caucasus, the center of origin for G. orientalis. All 101 isolates were characterized by genomic amplified fragment length polymorphism fingerprinting and by PCR-restriction fragment length polymorphism (RFLP) of the rRNA intergenic spacer and of five parts of the symbiotic region adjacent to nod box sequences. By all criteria, the R. galegae bv. officinalis and R. galegae bv. orientalis strains form distinct clusters. The nod box regions are highly conserved among strains belonging to each of the two biovars but differ structurally to various degrees between the biovars. The findings suggest varying evolutionary pressures in different parts of the symbiotic genome of closely related R. galegae biovars. Sixteen R. galegae bv. orientalis strains harbored copies of the same insertion sequence element; all were isolated from a particular site and belonged to a limited range of chromosomal genotypes. In all analyses, the Caucasian R. galegae bv. orientalis strains were more diverse than R. galegae bv. officinalis strains, in accordance with the gene center theory.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2003 American Society for Microbiology
Academic Units: The University of York > Biology (York)
Depositing User: Peter Young
Date Deposited: 28 Feb 2005
Last Modified: 17 Oct 2013 14:31
Published Version: http://dx.doi.org/10.1128/AEM.69.2.1067â¿¿1074....
Status: Published
Refereed: Yes
URI: http://eprints.whiterose.ac.uk/id/eprint/294

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