Weller, S.A., Stead, D.E. and Young, J.P.W. (2004) Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other -Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat. Applied and Environmental Microbiology. pp. 2779-2785. ISSN 0099-2240Full text available as:
Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi). Nine other pRi-harboring -Proteobacteria have subsequently been isolated from root mat-infected crops. Fatty acid profiling and partial 16S rRNA sequence analysis identified three of these strains as being in the genus Ochrobactrum, five as being in the genus Rhizobium, and one as being in the genus Sinorhizobium. An in vitro pathogenicity test involving inoculation of cucumber cotyledons was developed. All pRi-harboring -Proteobacteria induced typical root mat symptoms from the cotyledons. Average transformation rates for rhizogenic Ochrobactrum (46%) and Rhizobium (44%) strains were lower than those observed for rhizogenic A. radiobacter strains (64%). However, individual strains from these three genera all had transformation rates comparable to those observed from cotyledons inoculatedwith a rhizogenic Sinorhizobium strain (75%).
|Copyright, Publisher and Additional Information:||Copyright © 2004 American Society for Microbiology|
|Keywords:||MICROBIAL IDENTIFICATION SYSTEM, TI-PLASMID, RHIZOBIUM-MELILOTI, TRANSFORMED PLANTS, TAQMAN PCR, TUMEFACIENS, STRAINS, RHIZOGENES, SEQUENCE, RADIOBACTER|
|Academic Units:||The University of York > Biology (York)|
|Depositing User:||Peter Young|
|Date Deposited:||28 Feb 2005|
|Last Modified:||17 Oct 2013 14:28|