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Conservation of structure and mechanism in primary and secondary transporters exemplified by SiaP, a sialic acid binding virulence factor from Haemophilus influenzae

Muller, A., Severi, E., Mulligan, C., Watts, A.G., Kelly, D.J., Wilson, K.S., Wilkinson, A.J. and Thomas, G.H. (2006) Conservation of structure and mechanism in primary and secondary transporters exemplified by SiaP, a sialic acid binding virulence factor from Haemophilus influenzae. Journal of Biological Chemistry. pp. 22212-22222. ISSN 0021-9258

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Abstract

Extracytoplasmic solute receptors (ESRs) are important components of solute uptake systems in bacteria, having been studied extensively as parts of ATP binding cassette transporters. Herein we report the first crystal structure of an ESR protein from a functionally characterized electrochemical ion gradient-dependent secondary transporter. This protein, SiaP, forms part of a tripartite ATP-independent periplasmic transporter specific for sialic acid in Haemophilus influenzae. Surprisingly, the structure reveals an overall topology similar to ATP binding cassette ESR proteins, which is not apparent from the sequence, demonstrating that primary and secondary transporters can share a common structural component. The structure of SiaP in the presence of the sialic acid analogue 2,3-didehydro-2-deoxyN-acetylneuraminic acid reveals the ligand bound in a deep cavity with its carboxylate group forming a salt bridge with a highly conserved Arg residue. Sialic acid binding, which obeys simple bimolecular association kinetics as determined by stopped-flow fluorescence spectroscopy, is accompanied by domain closure about a hinge region and the kinking of an alpha-helix hinge component. The structure provides insight into the evolution, mechanism, and substrate specificity of ESR-dependent secondary transporters that are widespread in prokaryotes.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2006 by the American Society for Biochemistry and Molecular Biology. Uploaded in accordance with the publisher's self-archiving policy.
Keywords: CRYSTAL-STRUCTURE, RHODOBACTER-CAPSULATUS, TRAP TRANSPORTERS, LIGAND-BINDING, HEMAGGLUTININ-NEURAMINIDASE, HALOMONAS-ELONGATA, MOLECULAR-GRAPHICS, SERUM RESISTANCE, SUGAR-BINDING, PROTEIN DCTP
Academic Units: The University of York > Biology (York)
The University of York > Chemistry (York)
Depositing User: Gavin H Thomas
Date Deposited: 30 Jul 2007
Last Modified: 17 Oct 2013 14:18
Published Version: http://dx.doi.org/10.1074/jbc.M603463200
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/2587

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