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No evidence for killer sperm or other selective interactions between human spermatozoa in ejaculates of different males in vitro

Moore, H.D.M., Martin, M. and Birkhead, T.R. (1999) No evidence for killer sperm or other selective interactions between human spermatozoa in ejaculates of different males in vitro. Proceedings of the Royal Society Series B: Biological Sciences, 266 (1436). p. 2343. ISSN 1471-2954

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Abstract

This study examines one of the possible mechanisms of sperm competition, i.e. the kamikaze sperm hypothesis. This hypothesis states that sperm from different males interact to incapacitate each other in a variety of ways. We used ejaculates from human donors to compare mixes of semen in vitro from the same or different males. We measured the following parameters: (i) the degree of sperm aggregation, velocity and proportion of morphologically normal sperm after 1 and 3 h incubation in undiluted semen samples, (ii) the proportion of viable sperm plus the same parameters as in (i) in 'swim-up' sperm suspensions after 1 and 3 h incubation, (iii) the degree of self and non-self sperm aggregation using fluorescent dyes to distinguish the sperm of different males, and (iv) the extent of sperm capacitation and acrosome-reacted sperm in mixtures of sperm from the same and different males. We observed very few significant changes in sperm aggregation or performance in mixtures of sperm from different males compared with mixtures from the same male and none that were consistent with previously reported findings. The incapacitation of rival sperm therefore seems an unlikely mechanism of sperm competition in humans.

Item Type: Article
Copyright, Publisher and Additional Information: © 1999 The Royal Society
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield)
The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield)
The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine (Sheffield) > Clinical Sciences Division South (Sheffield) > Reproductive and Developmental Medicine (Sheffield)
Depositing User: Repository Officer
Date Deposited: 16 Nov 2004
Last Modified: 04 Jun 2014 13:24
Published Version: http://www.journals.royalsoc.ac.uk./openurl.asp?ge...
Status: Published
Refereed: Yes
Identification Number: 10.1098/rspb.1999.0929
URI: http://eprints.whiterose.ac.uk/id/eprint/197

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