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The crystal structure of superoxide dismutase from Plasmodium falciparum

Boucher, Ian W., Brzozowski, Andrzej M., Brannigan, James A., Schnick, Claudia, Smith, Derek J., Kyes, Sue A. and Wilkinson, Anthony J. (2006) The crystal structure of superoxide dismutase from Plasmodium falciparum. BMC Structural Biology. -. ISSN 1472-6807

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Abstract

Background: Superoxide dismutases (SODs) are important enzymes in defence against oxidative stress. In Plasmodium falciparum, they may be expected to have special significance since part of the parasite life cycle is spent in red blood cells where the formation of reactive oxygen species is likely to be promoted by the products of haemoglobin breakdown. Thus, inhibitors of P. falciparum SODs have potential as anti-malarial compounds. As a step towards their development we have determined the crystal structure of the parasite's cytosolic iron superoxide dismutase. Results: The cytosolic iron superoxide dismutase from P. falciparum (PfFeSOD) has been overexpressed in E. coli in a catalytically active form. Its crystal structure has been solved by molecular replacement and refined against data extending to 2.5 angstrom resolution. The structure reveals a two-domain organisation and an iron centre in which the metal is coordinated by three histidines, an aspartate and a solvent molecule. Consistent with ultracentrifugation analysis the enzyme is a dimer in which a hydrogen bonding lattice links the two active centres. Conclusion: The tertiary structure of PfFeSOD is very similar to those of a number of other iron-and manganese-dependent superoxide dismutases, moreover the active site residues are conserved suggesting a common mechanism of action. Comparison of the dimer interfaces of PfFeSOD with the human manganese-dependent superoxide dismutase reveals a number of differences, which may underpin the design of parasite-selective superoxide dismutase inhibitors.

Item Type: Article
Copyright, Publisher and Additional Information: © 2006 Boucher et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: MANGANESE, INTERFACE, SEQUENCE, INHIBITORS, IDENTIFICATION, DYNAMICS, ASSAYS
Institution: The University of York
Academic Units: The University of York > Chemistry (York)
Depositing User: Sherpa Assistant
Date Deposited: 24 Oct 2008 14:25
Last Modified: 14 Oct 2014 23:04
Published Version: http://dx.doi.org/10.1186/1472-6807-6-20
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/1873

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