Blood‑derived microRNAs (miRNAs/miRs) are ideal clinical biomarkers, as they can be relatively non‑invasively extracted and are stable across a range of storage conditions. However, the concentration and profile of miRNAs differ between specific patient groups and starting media, which must be a key consideration before embarking upon uses for clinical applications. The optimum blood‑derived starting media for biomarker discovery involving pregnant women with an uncomplicated pregnancy has not been determined. Paired serum and plasma samples were collected from 10 pregnant women with uncomplicated low‑risk pregnancies at three time points: i) During the second trimester of pregnancy; ii) during the third trimester; and iii) 6 weeks post‑partum. Sample miRNA content was assessed using an Agilent Bioanalyzer Small RNA chip and reverse transcription‑quantitative (RT‑q)PCR using four constitutively expressed miRNAs: hsa‑miR‑222‑3p, hsa‑miR‑23a, hsa‑miR‑30e‑5p and hsa‑miR‑451a. Quality control spike‑ins measured RNA extraction (UniSp2) and cDNA extraction (cel‑miR‑39‑3p) efficiency. MiRNA concentration and percentage were significantly higher in the serum vs. plasma samples based on data obtained from the Bioanalyzer; however, RT‑qPCR failed to replicate these differences in the majority of comparisons using the ΔCq values of the four constitutively expressed miRNAs. Using the standard deviations of the ΔCq values, the consistency of serum and plasma in terms of miRNA expression levels were equivalent. Thus, clinicians and researchers should take into consideration that different miRNA quantification methods can yield contrasting results with regards to the starting media utilized. Based on the equivalent performance of serum and plasma assessed using RT‑qPCR, which is less likely to be influenced by the coagulation process or degraded long RNAs, both starting media assessed in the present study are equally suitable for ongoing biomarker discovery studies involving healthy pregnant women at any gestational time point or immediately postpartum.
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)