Kirkby, CJ, Gala De Pablo, J, Tinkler-Hundal, E et al. (3 more authors) (2020) Developing a Raman spectroscopy-based tool to stratify patient response to pre-operative radiotherapy in rectal cancer. The Analyst. ISSN 0003-2654
Abstract
Rectal cancer patients frequently receive pre-operative radiotherapy (RT), prior to surgical resection. However, colorectal cancer is heterogeneous and the degree of tumour response to pre-operative RT is highly variable. There are currently no clinically approved methods of predicting response to RT, and a significant proportion of patients will show no clinical benefit, despite enduring the side-effects. We evaluated the use of Raman spectroscopy (RS), a non-destructive technique able to provide the unique chemical fingerprint of tissues, as a potential tool to stratify patient response to pre-operative RT. Raman measurements were obtained from the formalin-fixed, paraffin-embedded (FFPE) pre-treatment biopsy specimens of 20 rectal cancer patients who received pre-operative RT. A principal component analysis and linear discriminant analysis algorithm was able to classify patient response to pre-operative RT as good or poor, with an accuracy of 86.04 ± 0.14% (standard error). Patients with a good response to RT showed greater contributions from protein-associated peaks, whereas patients who responded poorly showed greater lipid contributions. These results demonstrate that RS is able to reliably classify tumour response to pre-operative RT from FFPE biopsies and highlights its potential to guide personalised cancer patient treatment.
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Copyright, Publisher and Additional Information: | © The Royal Society of Chemistry 2020. This is an open access article under the terms of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) | ||||||||
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Institution: | The University of Leeds | ||||||||
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) | ||||||||
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Depositing User: | Symplectic Publications | ||||||||
Date Deposited: | 13 Nov 2020 17:01 | ||||||||
Last Modified: | 25 Jun 2023 22:30 | ||||||||
Status: | Published online | ||||||||
Publisher: | Royal Society of Chemistry (RSC) | ||||||||
Identification Number: | https://doi.org/10.1039/d0an01803a | ||||||||
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Commentary/Response Threads
- Kirkby, CJ, Gala De Pablo, J, Tinkler-Hundal, E, Wood, HM, Evans, SD and West, NP Developing a Raman spectroscopy-based tool to stratify patient response to pre-operative radiotherapy in rectal cancer. (deposited 13 Nov 2020 17:01) [Currently Displayed]