Atkinson, F., Evans, R., Guest, J.E. et al. (4 more authors) (2020) Cyclical strain improves artificial equine tendon constructs in vitro. Journal of Tissue Engineering and Regenerative Medicine, 14 (5). pp. 690-700. ISSN 1932-6254
Abstract
Tendon injuries are a common cause of morbidity in humans. They also occur frequently in horses and the horse provides a relevant, large animal model in which to test novel therapies. To develop novel cell therapies that can aid tendon regeneration and reduce subsequent re‐injury rates, the mechanisms that control tendon tissue regeneration and matrix remodelling need to be better understood. Whilst a range of chemical cues have been explored (growth factors, media etc.), the influence of the mechanical environment on tendon cell culture has yet to be fully elucidated. To mimic the in vivo environment, in this study we have utilised a novel and affordable, custom‐made bioreactor to apply a cyclical strain to tendon‐like constructs generated in 3‐dimensional (3D) culture by equine tenocytes. Dynamic shear analysis (DSA), dynamic scanning calorimetry (DSC) and Fourier Transform Infrared (FTIR) spectroscopy were used to determine the mechanical and chemical properties of the resulting tendon‐like constructs. Our results demonstrate that equine tenocytes exposed to a 10% cyclical strain have an increased amount of collagen gel contraction after 7 and 8 days of culture compared to cells cultured in 3D in the absence of external strain. While all the tendon‐like constructs have a very similar chemical composition to native tendon, the application of strain improves their mechanical properties. We envisage these results will contribute towards the development of improved biomimetic artificial tendon models for the development of novel strategies for equine regenerative therapies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 John Wiley & Sons, Ltd. This is an author-produced version of a paper subsequently published in Journal of Tissue Engineering and Regenerative Medicine. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | 3D culture; tendon cells; dynamic shear analysis; mechanical properties; equine |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL EP/K005693/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 20 Mar 2020 10:16 |
Last Modified: | 03 Dec 2021 11:02 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/term.3030 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:158609 |