Exploiting nanobodies and Affimers for superresolution imaging in light microscopy

Abstract

Antibodies have long been the main approach used for localizing proteins of interest by light microscopy. In the past 5 yr or so, and with the advent of superresolution microscopy, the diversity of tools for imaging has rapidly expanded. One main area of expansion has been in the area of nanobodies, small single-chain antibodies from camelids or sharks. The other has been the use of artificial scaffold proteins, including Affimers. The small size of nanobodies and Affimers compared with the traditional antibody provides several advantages for superresolution imaging.

Metadata

Authors/Creators:

Carrington, G
Tomlinson, D https://orcid.org/0000-0003-4134-7484
Peckham, M https://orcid.org/0000-0002-3754-2028

© 2019 Carrington et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0)

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