El-Jawhari, J orcid.org/0000-0002-0580-4492, Ganguly, P, Churchman, S et al. (2 more authors) (2019) The biological fitness of bone progenitor cells in Reamer Irrigator Aspirator-waste. Journal of Bone and Joint Surgery, 101 (23). pp. 2111-2119. ISSN 0021-9355
Abstract
Background: The biological waste collected during use of the Reamer/Irrigator/Aspirator (RIA; DePuy Synthes) has been described as an abundant source of bone progenitor cells with a comparable osteogenic gene profile to donor-matched iliac crest bone marrow (IC-BM). However, it is not clear whether these RIA-waste (RIA-W) cells are biologically fit. We aimed to evaluate the stress levels and functions of RIA-W progenitor cells.
Methods: Reactive oxygen species (ROS) levels were tested in freshly collected bone progenitor cells (defined as CD45lowCD271high cells) using flow cytometry. ROS levels induced in these cells by hypoxia and/or oxidative stress as well as by an experimental simulation of the RIA procedure were also measured. Furthermore, the alkaline phosphatase (ALP) expression levels, proliferation, and senescence of culture-expanded RIA-W and IC-BM mesenchymal stromal cells (MSCs) were compared.
Results: RIA-W and donor-matched IC-BM CD45lowCD271high cells were 97% and 98% viable, but the ROS levels were significantly higher for RIA-W cells than for IC-BM cells (p = 0.0020). Also, ROS induced by hypoxia, oxidative stress, and both were higher for RIA-W cells (p = 0.0312, 0.0156, and 0.0703, respectively). Dilution with saline solution, suction pressure, and irrigation reduced cell viability, with a positive correlation with the ROS level (p = 0.0035). The RIA-W and IC-BM colony-forming cells (average, 96,100 and 11,500, respectively) showed comparable ALP levels. Furthermore, culture-expanded RIA-W and IC-BM MSCs showed comparable ROS levels, ALP levels, susceptibility to death, and proliferation.
Conclusions: Although freshly collected RIA-W bone progenitor cells appeared to be transiently stressed, these cells were as viable as IC-BM cells and present in greater numbers. The proliferation and osteogenesis of both cell types were comparable.
Clinical Relevance: The RIA waste bag contains bone progenitor cells with promising potential for regenerative applications, and should not be wasted.
Metadata
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Copyright, Publisher and Additional Information: | Copyright 2019 by The Journal of Bone and Joint Surgery, Incorporated. This is an author produced version of a paper published in The Journal of Bone and Joint Surgery. Uploaded in accordance with the publisher's self-archiving policy. | ||||||||
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Institution: | The University of Leeds | ||||||||
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) > Experimental Musculoskeletal Medicine (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) > Orthopaedics (Leeds) |
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Depositing User: | Symplectic Publications | ||||||||
Date Deposited: | 01 Oct 2019 10:52 | ||||||||
Last Modified: | 09 Oct 2020 00:38 | ||||||||
Status: | Published | ||||||||
Publisher: | The Journal of Bone and Joint Surgery, Incorporated | ||||||||
Identification Number: | https://doi.org/10.2106/JBJS.19.00133 |