Woodley, K.T. orcid.org/0000-0003-4002-5176 and Collins, M.O. orcid.org/0000-0002-7656-4975 (2019) Quantitative analysis of protein S-acylation site dynamics using site-specific acyl-biotin exchange (ssABE). In: Evans, C., Wright, P. and Noirel, J., (eds.) Mass Spectrometry of Proteins. Methods in Molecular Biology, 1977 . Humana Press , New York , pp. 71-82. ISBN 9781493992317
Abstract
Protein S-acylation (palmitoylation) is a reversible lipid modification that is increasingly recognized as an important regulator of protein function, including membrane association, trafficking, and subcellular localization. Most proteomic methods to study palmitoylation allow characterization of putative palmitoylated proteins but do not permit identification of individual sites of palmitoylation. We have recently adapted the Acyl-Biotin Exchange (ABE) method that is routinely used for palmitoyl-proteome characterization, to permit global S-acylation site analysis. This site-specific ABE (ssABE) protocol, when combined with SILAC-based quantification, allows both the large-scale identification of palmitoylation sites and quantitative profiling of palmitoylation site changes. This approach enables palmitoylation to be studied at a systems level comparable to other more intensively studied post-translational modifications.
Metadata
Item Type: | Book Section |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © Springer Science+Business Media, LLC, part of Springer Nature 2019. This is an author-produced version of a chapter subsequently published in Evans C., Wright P., Noirel J. (eds) Mass Spectrometry of Proteins. Methods in Molecular Biology, vol 1977. Humana Press, New York, NY. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Mass spectrometry; Membrane; Palmitoylation; Protein; Proteomic; Quantitative; S-Acylation |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 16 May 2019 14:13 |
Last Modified: | 13 Apr 2021 00:38 |
Status: | Published |
Publisher: | Humana Press |
Series Name: | Methods in Molecular Biology |
Refereed: | Yes |
Identification Number: | 10.1007/978-1-4939-9232-4_6 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:145482 |