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Rapid Detection of Leishmania infantum Infection in Dogs: Comparative Study Using an Immunochromatographic Dipstick Test, Enzyme-Linked Immunosorbent Assay, and PCR

Reithinger, R., Quinnell, R.J., Alexander, B. and Davies, C.R. (2002) Rapid Detection of Leishmania infantum Infection in Dogs: Comparative Study Using an Immunochromatographic Dipstick Test, Enzyme-Linked Immunosorbent Assay, and PCR. Journal of Clinical Microbiology, 40 (7). 2352-2356. ISSN 1098-660X

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Abstract

Current zoonotic visceral leishmaniasis (ZVL) control programs in Brazil include the culling of Leishmania infantum-infected reservoir dogs, a strategy that has failed to prevent a rise of canine and human ZVL cases over the past decade. One of the main reasons this strategy has failed is because of a long delay between sample collection, sample analysis, and control implementation. A rapid, sensitive, and specific diagnostic tool would be highly desirable, because it would allow control interventions to be implemented in situ. We compared an immunochromatographic dipstick test to enzyme-linked immunosorbent assay (ELISA) and PCR for detecting L. infantum infections in dogs from an area of ZVL endemicity in Brazil. The dipstick test was shown to have 61 to 75% specificity and 72 to 77% sensitivity, compared to 100% specificity for both ELISA and PCR and 71 to 88% and 51 to 64% sensitivity for ELISA and PCR, respectively. Of the field samples tested, 92 of 175 (53%), 65 of 175 (37%), and 47 of 175 (27%) were positive by dipstick, ELISA, and PCR, respectively. The positive and negative predictive values for the tested dipstick were 58 to 77% and 75%, respectively. Efforts should be made to develop a more specific dipstick test for diagnosis of leishmaniasis, because they may ultimately prove more cost-effective than currently used diagnostic tests when used in mass-screening surveys.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2002, American Society for Microbiology. Reproduced in accordance with the publisher's self-archiving policy.
Academic Units: The University of Leeds > Faculty of Biological Sciences (Leeds) > Institute of Integrative and Comparative Biology (Leeds)
Depositing User: Repository Officer
Date Deposited: 26 Jun 2006
Last Modified: 08 Feb 2013 17:02
Published Version: http://dx.doi.org/10.1128/JCM.40.7.2352-2356.2002
Status: Published
Publisher: American Society for Microbiology
Refereed: Yes
Identification Number: 10.1128/JCM.40.7.2352-2356.2002
URI: http://eprints.whiterose.ac.uk/id/eprint/1380

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