Near-field ptychographic microscope for quantitative phase imaging

Quantitative phase imaging (QPI) is the name given to a set of microscopy techniques that map out variations in optical path lengths across a sample. These maps are a useful source of contrast for transparent samples such as biological cells, and because they are quantitative they can be used to measure refractive index and thickness variations. Here we detail the setup and operation of a new form of QPI microscope based on near-field ptychography. We test our system using a range of phase objects, and analyse the phase images it produces. Our results show that accurate, high quality images can be obtained from a ptychographical dataset containing as few as four near-field diffraction patterns. We also assess how our system copes with optically thick samples and samples with a wide range of spatial frequencies – two areas where conventional and Fourier ptychography struggle.