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A five year outbreak of methicillin-susceptible Staphylococcus aureus phage type 53,85 in a regional neonatal unit

Wilcox, M.H., Fitzgerald, P., Freeman, J., Denton, M., Gill, A.B., Hoy, C., Parnell, P., Porter, C., Haspinall, L. and Hawkey, P. (2000) A five year outbreak of methicillin-susceptible Staphylococcus aureus phage type 53,85 in a regional neonatal unit. Epidemiology and Infection, 124 (1). pp. 37-45. ISSN 0950-2688

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Abstract

We identified a 5-year outbreak of a methicillin-susceptible Staphylococcus aureus (MSSA) strain, affecting 202 babies on a neonatal unit, by routine weekly phage typing all S. aureus isolates. Multiple staged control measures including strict emphasis on hand hygiene, environmental and staff surveillance sampling, and application of topical hexachlorophane powder failed to end the outbreak. S. aureus PT 53,85 (SA5385) was found on opened packs of Stomahesive®, used as a neonatal skin protectant.

Only following the implementation of aseptic handling of Stomahesive®, and the use of topical mupirocin for staff nasal carriers of SA5385, and for babies colonized or infected with S. aureus, did the isolation rate of SA5385 decline. DNA fingerprinting indicated that [gt-or-equal, slanted]95% of SA5385 isolates were clonal. In vitro death rates of SA5385 on Stomahesive® with human serum were significantly lower than on Stomahesive® alone (P = 0·04), and on cotton sheet with serum (P = 0·04), highlighting the potential of this material as a survival niche. Phage typing remains a valuable, inexpensive and simple method for monitoring nosocomial MSSA infection.

Item Type: Article
Copyright, Publisher and Additional Information: © 2000 Cambridge University Press
Institution: The University of Leeds
Academic Units: The University of Leeds > Faculty of Biological Sciences (Leeds) > Institute of Molecular and Cellular Biology (Leeds)
Depositing User: Repository Assistant
Date Deposited: 15 Jun 2006
Last Modified: 05 Jun 2014 01:29
Published Version: http://dx.doi.org/10.1017/S0950268899003349
Status: Published
Publisher: Cambridge University Press
Refereed: Yes
Identification Number: 10.1017/S0950268899003349
URI: http://eprints.whiterose.ac.uk/id/eprint/1292

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